#!/bin/bash

# inputs
in_fasta="$1"
out_dir="$2"

# resources
CPU="$3"
MEM="$4"

# pipe_dir
PIPE_DIR="$5"
DB_TEMPL="$6"

# sequence databases
DB_UR30="$PIPE_DIR/uniclust/UniRef30_2021_06"
DB_BFD="$PIPE_DIR/bfd/bfd_metaclust_clu_complete_id30_c90_final_seq.sorted_opt"

# Running signalP 6.0
mkdir -p $out_dir/signalp
tmp_dir="$out_dir/signalp"
signalp6 --fastafile $in_fasta --organism other --output_dir $tmp_dir --format none --mode slow
trim_fasta="$tmp_dir/processed_entries.fasta"
if [ ! -s $trim_fasta ] # empty file -- no signal P
then
    trim_fasta="$in_fasta"
fi

# setup hhblits command
export HHLIB=/software/hhsuite/build/bin/
export PATH=$HHLIB:$PATH
HHBLITS_UR30="hhblits -o /dev/null -mact 0.35 -maxfilt 100000000 -neffmax 20 -cov 25 -cpu $CPU -nodiff -realign_max 100000000 -maxseq 1000000 -maxmem $MEM -n 4 -d $DB_UR30"
HHBLITS_BFD="hhblits -o /dev/null -mact 0.35 -maxfilt 100000000 -neffmax 20 -cov 25 -cpu $CPU -nodiff -realign_max 100000000 -maxseq 1000000 -maxmem $MEM -n 4 -d $DB_BFD"

mkdir -p $out_dir/hhblits
tmp_dir="$out_dir/hhblits"
out_prefix="$out_dir/t000_"

# perform iterative searches against UniRef30
if [ ! -s ${out_prefix}.msa0.a3m ]
then
    prev_a3m="$trim_fasta"
    for e in 1e-10 1e-6 1e-3
    do
        echo "Running HHblits against UniRef30 with E-value cutoff $e"
        if [ ! -s $tmp_dir/t000_.$e.a3m ]
        then
            $HHBLITS_UR30 -i $prev_a3m -oa3m $tmp_dir/t000_.$e.a3m -e $e -v 0
        fi
        hhfilter -maxseq 100000 -id 90 -cov 75 -i $tmp_dir/t000_.$e.a3m -o $tmp_dir/t000_.$e.id90cov75.a3m
        hhfilter -maxseq 100000 -id 90 -cov 50 -i $tmp_dir/t000_.$e.a3m -o $tmp_dir/t000_.$e.id90cov50.a3m
        prev_a3m="$tmp_dir/t000_.$e.id90cov50.a3m"
        n75=`grep -c "^>" $tmp_dir/t000_.$e.id90cov75.a3m`
        n50=`grep -c "^>" $tmp_dir/t000_.$e.id90cov50.a3m`

        if ((n75>2000))
        then
            if [ ! -s ${out_prefix}.msa0.a3m ]
            then
                cp $tmp_dir/t000_.$e.id90cov75.a3m ${out_prefix}.msa0.a3m
                break
            fi
        elif ((n50>4000))
        then
            if [ ! -s ${out_prefix}.msa0.a3m ]
            then
                cp $tmp_dir/t000_.$e.id90cov50.a3m ${out_prefix}.msa0.a3m
                break
            fi
        else
            continue
        fi
    done

    # perform iterative searches against BFD if it failes to get enough sequences
    if [ ! -s ${out_prefix}.msa0.a3m ] 
    then
        e=1e-3
        echo "Running HHblits against BFD with E-value cutoff $e"
        if [ ! -s $tmp_dir/t000_.$e.bfd.a3m ]
        then
            $HHBLITS_BFD -i $prev_a3m -oa3m $tmp_dir/t000_.$e.bfd.a3m -e $e -v 0
        fi
        hhfilter -maxseq 100000 -id 90 -cov 75 -i $tmp_dir/t000_.$e.bfd.a3m -o $tmp_dir/t000_.$e.bfd.id90cov75.a3m
        hhfilter -maxseq 100000 -id 90 -cov 50 -i $tmp_dir/t000_.$e.bfd.a3m -o $tmp_dir/t000_.$e.bfd.id90cov50.a3m
        prev_a3m="$tmp_dir/t000_.$e.bfd.id90cov50.a3m"
        n75=`grep -c "^>" $tmp_dir/t000_.$e.bfd.id90cov75.a3m`
        n50=`grep -c "^>" $tmp_dir/t000_.$e.bfd.id90cov50.a3m`

        if ((n75>2000))
        then
            if [ ! -s ${out_prefix}.msa0.a3m ]
            then
                cp $tmp_dir/t000_.$e.bfd.id90cov75.a3m ${out_prefix}.msa0.a3m
            fi
        elif ((n50>4000))
        then
            if [ ! -s ${out_prefix}.msa0.a3m ]
            then
                cp $tmp_dir/t000_.$e.bfd.id90cov50.a3m ${out_prefix}.msa0.a3m
            fi
        fi
    fi

    if [ ! -s ${out_prefix}.msa0.a3m ]
    then
        cp $prev_a3m ${out_prefix}.msa0.a3m
    fi
fi
    
echo "Running PSIPRED"
$PIPE_DIR/input_prep/make_ss.sh $out_dir/t000_.msa0.a3m $out_dir/t000_.ss2 > $out_dir/log/make_ss.stdout 2> $out_dir/log/make_ss.stderr

if [ ! -s $out_dir/t000_.hhr ]
then
    echo "Running hhsearch"
    HH="hhsearch -b 50 -B 500 -z 50 -Z 500 -mact 0.05 -cpu $CPU -maxmem $MEM -aliw 100000 -e 100 -p 5.0 -d $DB_TEMPL"
    
    cat $out_dir/t000_.ss2 $out_dir/t000_.msa0.a3m > $out_dir/t000_.msa0.ss2.a3m
    $HH -i $out_dir/t000_.msa0.ss2.a3m -o $out_dir/t000_.hhr -atab $out_dir/t000_.atab -v 0
fi